anti- MAPKAPK2 antibody

anti- MAPKAPK2 antibody

Product Name

MAPKAPK2 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSF1, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to the dissociation of HSP27/HSPB1 from large small heat-shock protein(sHsps) oligomers and impairment of their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor(TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements(AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to the regulation of the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity, leading to inhibition of dependent degradation of ARE-containing transcripts. Phosphorylates CEP131 in response to cellular stress induced by ultraviolet irradiation which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites(PubMed:26616734). Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA. Involved in toll-like receptor signaling pathway(TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.

IMMUNOGEN INFORMATION

Immunogen

mitogen-activated protein kinase-activated protein kinase 2

Synonyms

MAPKAP kinase 2, MAPKAPK 2, MAPKAPK2, MK2

Observed MW

47-50 kDa

APPLICATION

Tested Application

ELISA, WB, IHC

Recommended Dilution

WB: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

P49137

IMAGES

Immunohistochemistry of paraffin-embedded human kidney tissue slide using FNab04998(MAPKAPK2 Antibody) at dilution of 1:50

HeLa cells were subjected to SDS PAGE followed by western blot with FNab04998(MAPKAPK2 antibody) at dilution of 1:1000

RELATED PRODUCTS

• Human MAPKAPK2(MAP kinase-activated protein kinase 2) ELISA Kit
• Human LRWD1(Leucine-rich repeat and WD repeat-containing protein 1) ELISA Kit

STATEMENT

Manuals on the web are for your reference only, specifications are subject to the delivery manuals.

FEATURED PRODUCT