anti- MAVS; VISA antibody

anti- MAVS; VISA antibody

Product Name

MAVS; VISA antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Required for innate immune defense against viruses. Acts downstream of DDX58/RIG-I and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES(CCL5). Peroxisomal and mitochondrial MAVS act sequentially to create an antiviral cellular state. Upon viral infection, peroxisomal MAVS induces the rapid interferon-independent expression of defense factors that provide short-term protection, whereas mitochondrial MAVS activates an interferon-dependent signaling pathway with delayed kinetics, which amplifies and stabilizes the antiviral response. May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis.It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.

IMMUNOGEN INFORMATION

Immunogen

mitochondrial antiviral signaling protein

Synonyms

CARD adapter inducing interferon beta,Interferon beta promoter stimulator protein 1,Cardif,Putative NF-kappa-B-activating protein 031N,Virus-induced-signaling adapter

Observed MW

70 kDa

APPLICATION

Tested Application

ELISA, WB, IF, IP, IHC

Recommended Dilution

WB: 1:1000-1:4000; IP: 1:500-1:2000; IHC: 1:100-1:500; IF: 1:10-1:100

UNIPROT INFORMATION

UniProt ID

Q7Z434

IMAGES

Immunohistochemistry of paraffin-embedded human heart tissue slide using FNab05032(MAVS Antibody) at dilution of 1:100

IP Result of anti-MAVS; VISA (IP:FNab05032, 3ug; Detection:FNab05032 1:1000) with HEK-293 cells lysate 1700ug.

HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab05032(MAVS antibody) at dilution of 1:2000