anti- MBP tag antibody

Product Name

MBP tag antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG2a

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin.

IMMUNOGEN INFORMATION

Immunogen

MBP-Tag

Synonyms

Maltose Binding Protein, MBP, MBP Tag

Observed MW

40 kDa

APPLICATION

Tested Application

ELISA, WB, IP

Recommended Dilution

WB: 1:1000-1:8000; IP: 1:5000-1:50000

IMAGES

IP Result of anti-MBP tag (IP: FNab05042, 5ug; Detection: FNab05042 1:20000) with Recombinant protein protein lysate 800ug.

Recombinant protein were subjected to SDS PAGE followed by western blot with FNab05042(MBP tag antibody) at dilution of 1:4000