anti- MCM5 antibody

anti- MCM5 antibody

Product Name

MCM5 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Acts as component of the MCM2-7 complex(MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity(By similarity). Interacts with MCMBP.

IMMUNOGEN INFORMATION

Immunogen

minichromosome maintenance complex component 5

Synonyms

CDC46

Observed MW

82 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P33992

IMAGES

Immunohistochemistry of paraffin-embedded human lymphoma using FNab05060(MCM5 antibody) at dilution of 1:100

IP Result of anti-MCM5 (IP:FNab05060, 4ug; Detection:FNab05060 1:500) with Jurkat cells lysate 2400ug.

Jurkat cells were subjected to SDS PAGE followed by western blot with FNab05060(MCM5 antibody) at dilution of 1:500