anti- MDM2 antibody

anti- MDM2 antibody

Product Name

MDM2 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

BACKGROUND

This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.

IMMUNOGEN INFORMATION

Immunogen

Mdm2 p53 binding protein homolog ( Mouse )

Synonyms

ACTFS

Observed MW

50 kDa

APPLICATION

Tested Application

ELISA, IHC, WB, IF

Recommended Dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200

UNIPROT INFORMATION

UniProt ID

Q00987

IMAGES

Product Name

MDM2 antibody

Catalogue No.

FNab05077

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

BACKGROUND

This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.

IMMUNOGEN INFORMATION

Immunogen

Mdm2 p53 binding protein homolog ( Mouse )

Synonyms

ACTFS

Observed MW

50 kDa

APPLICATION

Tested Application

ELISA, IHC, WB, IF

Recommended Dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200

UNIPROT INFORMATION

UniProt ID

Q00987

IMAGES

Immunohistochemistry of paraffin-embedded human stomach using FNab05077(MDM2 antibody) at dilution of 1:100

HT-29 cells were subjected to SDS PAGE followed by western blot with FNab05077(MDM2 antibody) at dilution of 1:1000