anti- MED15 antibody

anti- MED15 antibody

Product Name

MED15 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway.

IMMUNOGEN INFORMATION

Immunogen

mediator complex subunit 15

Synonyms

ARC105, CTG7A, PCQAP, TIG1, TNRC7

Observed MW

105 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q96RN5

IMAGES

Immunohistochemistry of paraffin-embedded human prostate cancer using FNab05089(MED15 antibody) at dilution of 1:100

IP Result of anti-MED15 (IP:FNab05089, 3ug; Detection:FNab05089 1:500) with K-562 cells lysate 3440ug.

K-562 cells were subjected to SDS PAGE followed by western blot with FNab05089(MED15 antibody) at dilution of 1:500