anti- MGEA5 antibody

anti- MGEA5 antibody

Product Name

MGEA5 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc(in vitro)(PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity(PubMed:24088714). Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc(in vitro), but has about six times lower specific activity than isoform 1.

IMMUNOGEN INFORMATION

Immunogen

meningioma expressed antigen 5(hyaluronidase)

Synonyms

HEXC, KIAA0679, MEA5

Observed MW

130 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

O60502

IMAGES

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using FNab05163(MGEA5 Antibody) at dilution of 1:200

IP Result of anti-MGEA5 (IP:FNab05163, 3ug; Detection:FNab05163 1:500) with SH-SY5Y cells lysate 2900ug.

BxPC-3 cells were subjected to SDS PAGE followed by western blot with FNab05163(MGEA5 Antibody) at dilution of 1:1500