anti- MGEA5 antibody

anti- MGEA5 antibody

Product Name

MGEA5 antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG2a

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc(in vitro)(PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity(PubMed:24088714). Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc(in vitro), but has about six times lower specific activity than isoform 1.

IMMUNOGEN INFORMATION

Immunogen

meningioma expressed antigen 5(hyaluronidase)

Synonyms

HEXC, KIAA0679, MEA5

Observed MW

130 kDa

APPLICATION

Tested Application

ELISA, WB, IF, IHC

Recommended Dilution

WB: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

O60502

IMAGES

Immunohistochemistry of paraffin-embedded human gliomas using FNab05164(MGEA5 antibody) at dilution of 1:50

Human brain lysate were subjected to SDS PAGE followed by western blot with FNab05164(MGEA5 antibody) at dilution of 1:600