anti- MGMT antibody

anti- MGMT antibody

Product Name

MGMT antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Involved in the cellular defense against the biological effects of O6-methylguanine(O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

IMMUNOGEN INFORMATION

Immunogen

O-6-methylguanine-DNA methyltransferase

Synonyms

MGMT

Observed MW

22 kDa

APPLICATION

Tested Application

ELISA, WB, IHC

Recommended Dilution

WB: 1:500-1:5000; IP: 1:200-1:2000; IHC: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P16455

IMAGES

Immunohistochemistry of paraffin-embedded human kidney using FNab05169(MGMT antibody) at dilution of 1:100

Jurkat cells were subjected to SDS PAGE followed by western blot with FNab05169(MGMT antibody) at dilution of 1:1000