FineTest
SKU:FNab05187
anti- MIF antibody
anti- MIF antibody
Couldn't load pickup availability
anti- MIF antibody
Product Name
MIF antibody
Size
100µg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Host
Rabbit
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
BACKGROUND
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity(in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
IMMUNOGEN INFORMATION
Immunogen
macrophage migration inhibitory factor(glycosylation-inhibiting factor)
Synonyms
GIF, GLIF, L dopachrome isomerase, L dopachrome tautomerase, MIF, MMIF, Phenylpyruvate tautomerase
Observed MW
12 kDa
APPLICATION
Tested Application
ELISA, IHC, WB, IP, IF
Recommended Dilution
WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:50-1:500; IF: 1:50-1:500
UNIPROT INFORMATION
UniProt ID
IMAGES
Immunohistochemistry of paraffin-embedded human testis tissue slide using FNab05187(MIF Antibody) at dilution of 1:200
Immunofluorescent analysis of ( -20°C Ethanol) fixed A549 cells using FNab05187 (MIF antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L)
IP Result of anti-MIF (IP:FNab05187, 4ug; Detection:FNab05187 1:1000) with mouse spleen tissue lysate 4000ug.
Y79 cells were subjected to SDS PAGE followed by western blot with FNab05187(MIF antibody) at dilution of 1:1000
RELATED PRODUCTS
STATEMENT
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
FEATURED PRODUCT
