anti- MPRIP antibody

anti- MPRIP antibody

Product Name

MPRIP antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.

IMMUNOGEN INFORMATION

Immunogen

myosin phosphatase Rho interacting protein

Synonyms

KIAA0864, MRIP, RHOIP3

Observed MW

116-140 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF, IP

Recommended Dilution

WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:10-1:100

UNIPROT INFORMATION

UniProt ID

Q6WCQ1

IMAGES

Immunohistochemistry of paraffin-embedded human ovary using FNab05296(MPRIP antibody) at dilution of 1:100

Immunofluorescent analysis of Hela cells, using FNab05296(MPRIP antibody) at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

IP Result of anti-MPRIP (IP:FNab05296, 4ug; Detection:FNab05296 1:500) with SKOV-3 cells lysate 2000ug.

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with FNab05296(MPRIP antibody) at dilution of 1:800