anti- MSH2 antibody

anti- MSH2 antibody

Product Name

MSH2 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Component of the post-replicative DNA mismatch repair system(MMR). Forms two different heterodimers: MutS alpha(MSH2-MSH6 heterodimer) and MutS beta(MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops(IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

IMMUNOGEN INFORMATION

Immunogen

mutS homolog 2, colon cancer, nonpolyposis type 1(E. coli)

Synonyms

COCA1, FCC1, hMSH2, HNPCC, HNPCC1, LCFS2, MSH2, MutS protein homolog 2

Observed MW

105 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF, FC, IP

Recommended Dilution

WB: 1:1000-1:4000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:10-1:100

UNIPROT INFORMATION

UniProt ID

P43246

IMAGES

Immunohistochemistry of paraffin-embedded human colon cancer using FNab05371(MSH2 antibody) at dilution of 1:100

IP Result of anti-MSH2 (IP: FNab05371, 4ug; Detection: FNab05371 1:500) with HeLa cells lysate 2000ug.

HeLa cells were subjected to SDS PAGE followed by western blot with FNab05371(MSH2 antibody) at dilution of 1:1000