anti- MTF2 antibody

anti- MTF2 antibody

Product Name

MTF2 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Polycomb group(PcG) that specifically binds histone H3 trimethylated at 'Lys-36'(H3K36me3) and recruits the PRC2 complex. Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity. Regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation. Promotes recruitment of the PRC2 complex to the inactive X chromosome in differentiating XX ES cells and PRC2 recruitment to target genes in undifferentiated ES cells. Required to repress Hox genes by enhancing H3K27me3 methylation of the PRC2 complex. In some conditions may act as an inhibitor of PRC2 activity: able to activate the CDKN2A gene and promote cellular senescence by suppressing the catalytic activity of the PRC2 complex locally. Binds to the metal-regulating-element(MRE) of MT1A gene promoter(By similarity).

IMMUNOGEN INFORMATION

Immunogen

metal response element binding transcription factor 2

Synonyms

hPCl2, M96, MTF2, PCL2, Polycomb like protein 2, RP5 976O13.1

Observed MW

60-61 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF, IP

Recommended Dilution

WB: 1:500-1:1000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:50-1:200

UNIPROT INFORMATION

UniProt ID

Q9Y483

IMAGES

Immunohistochemistry of paraffin-embedded human brain using FNab05397(MTF2 antibody) at dilution of 1:50

IP Result of anti-MTF2 (IP:FNab05397, 4ug; Detection:FNab05397 1:300) with mouse skeletal muscle tissue lysate 4000ug.

Jurkat cells were subjected to SDS PAGE followed by western blot with FNab05397(MTF2 antibody) at dilution of 1:600