anti- MYC-tag antibody

anti- MYC-tag antibody

Product Name

MYC-tag antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino-or carboxy-terminus of targeted proteins.

IMMUNOGEN INFORMATION

Immunogen

MYC-tag

Synonyms

myc tag

APPLICATION

Tested Application

ELISA, WB, IP, IF

Recommended Dilution

WB: 1:1000-1:8000; IP: 1:500-1:2000 ; IF: 1:10-1:100

IMAGES

IP Result of anti-MYC-tag (IP: FNab05462, 4ug; Detection: FNab05462 1:1000) with Transfected HEK-293 cells lysate 300ug.

Myc-tagged fusion protein were subjected to SDS PAGE followed by western blot with FNab05462(MYC tag antibody) at dilution of 1:4000