anti- MYO9B antibody

anti- MYO9B antibody

Product Name

MYO9B antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Binds actin with high affinity both in the absence and presence of ATP and its mechanochemical activity is inhibited by calcium ions(PubMed:9490638). Also acts as a GTPase activator for RHOA(PubMed:9490638, PubMed:26529257). Plays a role in the regulation of cell migration via its role as RHOA GTPase activator. This is regulated by its interaction with the SLIT2 receptor ROBO1; interaction with ROBO1 impairs interaction with RHOA and subsequent activation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA(PubMed:26529257).

IMMUNOGEN INFORMATION

Immunogen

myosin IXB

Synonyms

MYR5

Observed MW

230-250kd

APPLICATION

Tested Application

ELISA, WB, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:200-1:2000

UNIPROT INFORMATION

UniProt ID

Q13459

IMAGES

IP Result of anti-MYO9B (IP:FNab05509, 5ug; Detection:FNab05509 1:500) with HepG2 cells lysate 2800ug.

mouse thymus tissue were subjected to SDS PAGE followed by western blot with FNab05509( MYO9B Antibody) at dilution of 1:600