anti- NAGA antibody

anti- NAGA antibody

Product Name

NAGA antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

NAGA belongs to the glycosyl hydrolase 27 family. It removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. It is required for the breakdown of glycolipids. Biosynthetic studies performed with cultured fibroblasts indicated that the human enzyme was synthesized as a 65kDa glycosylated precursor which was processed to a mature 48-kDa lysosomal form; both the precursor and mature forms had high mannose type oligosaccharide chains, but only the precursor’s mannose residues were phosphorylated. 90-117kd is a homodimer of NAGA.

IMMUNOGEN INFORMATION

Immunogen

N-acetylgalactosaminidase, alpha-

Synonyms

Alpha galactosidase B, D22S674, GALB, NAGA

Observed MW

48 kDa, 60-70 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF

Recommended Dilution

WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P17050

IMAGES

Immunohistochemistry of paraffin-embedded human placenta using FNab05537(NAGA antibody) at dilution of 1:100

HeLa cells were subjected to SDS PAGE followed by western blot with FNab05537(NAGA antibody) at dilution of 1:1000