anti- NCK1 antibody

anti- NCK1 antibody

Product Name

NCK1 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors.

IMMUNOGEN INFORMATION

Immunogen

NCK adaptor protein 1

Synonyms

NCK

Observed MW

43kd

APPLICATION

Tested Application

ELISA, WB, IHC, IF, FC, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P16333

IMAGES

Immunohistochemistry of paraffin-embedded human breast cancer using FNab05583(NCK1 antibody) at dilution of 1:100

IP Result of anti-NCK1 (IP:FNab05583, 4ug; Detection:FNab05583 1:500) with mouse brain tissue lysate 2640ug.

mouse brain tissue were subjected to SDS PAGE followed by western blot with FNab05583(NCK1 antibody) at dilution of 1:400