anti- NEK2 antibody

PRODUCT INFORMATION

Product Name

NEK2 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

BACKGROUND

This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene.

IMMUNOGEN INFORMATION

Immunogen

NIMA (never in mitosis gene a)-related kinase 2

Synonyms

NEK2A, NLK1

Observed MW

52 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF

Recommended Dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200

UNIPROT INFORMATION

UniProt ID

P51955

IMAGES

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using FNab05651 (NEK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)

K-562 cells were subjected to SDS PAGE followed by western blot with FNab05651(NEK2 antibody) at dilution of 1:1000