anti- NGEF antibody

PRODUCT INFORMATION

Product Name

NGEF antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Acts as a guanine nucleotide exchange factor(GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1-and CDC42-stimulated growth cone extension(By similarity).

IMMUNOGEN INFORMATION

Immunogen

neuronal guanine nucleotide exchange factor

Observed MW

75 kDa

APPLICATION

Tested Application

ELISA, WB, IP, IHC

Recommended Dilution

WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

Q8N5V2

IMAGES

IP Result of anti-NGEF (IP:FNab05720, 4ug; Detection:FNab05720 1:500) with mouse brain tissue lysate 4000ug.

mouse brain tissue were subjected to SDS PAGE followed by western blot with FNab05720(NGEF antibody) at dilution of 1:500