anti- Osteopontin antibody

PRODUCT INFORMATION

Product Name

Osteopontin antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Osteopontin(OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein(SIBLING) family. Originally isolated from bone, OPN has been found in kidney, vascular tissues, biological fluids, and various tumor tissues(PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression.

IMMUNOGEN INFORMATION

Immunogen

secreted phosphoprotein 1

Synonyms

BNSP, Bone sialoprotein 1, BSPI, ETA 1, Nephropontin, OPN, Osteopontin, secreted phosphoprotein 1, SPP 1, SPP1, Urinary stone protein, Uropontin

Observed MW

66 kDa

APPLICATION

Tested Application

ELISA, IHC, WB, IF

Recommended Dilution

WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P10451

Immunofluorescent analysis of HepG2 cells using FNab06033 (Osteopontin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)

mouse kidney tissue were subjected to SDS PAGE followed by western blot with FNab06033(OPN Antibody) at dilution of 1:1000