anti- p65/RELA antibody

PRODUCT INFORMATION

Product Name

p65/RELA antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Nuclear factor k B(NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-Kb family in mammalian cells include the proto-oncogene c-Rel,p50/p105(NFkB1), p65(RelA), p52/p100(NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-Kb. The p65 subunit is a major component of NF-Kb complexes and is responsible for trans-activation. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. It associates with chromatin at the NF-kappa-B promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.

IMMUNOGEN INFORMATION

Immunogen

v-rel reticuloendotheliosis viral oncogene homolog A(avian)

Synonyms

NFKB 3, NFKB p65, NFKB3, p65, RELA, Transcription factor p65

Observed MW

65 kDa

APPLICATION

Tested Application

ELISA, IHC, IF, IP, WB, FC

Recommended Dilution

WB: 1:500-1:5000; IP: 1:500-1:5000; IHC: 1:50-1:200; IF: 1:10-1:100

UNIPROT INFORMATION

UniProt ID

Q04206

HeLa cells were subjected to SDS PAGE followed by western blot with FNab06089(p65 antibody) at dilution of 1:1000