anti- PARD3 antibody

PRODUCT INFORMATION

Product Name

PARD3 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Involved in Schwann cell peripheral myelination(By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.PARD3 has three splice isoforms at 100 kDa, 150 kDa, and 180 kDa. This polyclonal antibody raised against C-terminal 281 amino acids of human PARD3 recognizes these three isoforms.

IMMUNOGEN INFORMATION

Immunogen

Partitioning defective 3 homolog

Synonyms

ASIP, Baz, Bazooka, CTCL tumor antigen se2 5, FLJ21015, PAR 3, PAR3, PAR3 alpha, PAR3A, PAR3alpha, PARD 3, PARD3, PARD3A, SE2 5L16, SE2 5LT1, SE2 5T2

Observed MW

100 kDa, 150 kDa, and 180 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, FC, IP

Recommended Dilution

WB: 1:500-1:1000; IP: 1:200-1:1000; IHC: 1:50-1:200; IF: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

Q8TEW0

Immunofluorescent analysis of fixed MCF-7 cells using FNab06146 (PARD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)

IP Result of anti-PARD3 (IP:FNab06146, 4ug; Detection:FNab06146 1:200) with MCF-7 cells lysate 2900ug.

MCF7 cells were subjected to SDS PAGE followed by western blot with FNab06146(PARD3 antibody) at dilution of 1:500