anti- PCNA antibody

PRODUCT INFORMATION

Product Name

PCNA antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic(AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response(DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways(PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion(TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

IMMUNOGEN INFORMATION

Immunogen

proliferating cell nuclear antigen

Synonyms

Proliferating cell nuclear antigen, Cyclin

Observed MW

38 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, FC, IP

Recommended Dilution

WB: 1:5000-1:50000; IP: 1:200-1:1000; IHC: 1:500-1:2000; IF: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

P12004

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using FNab06217 (PCNA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L)

IP Result of anti-PCNA (IP:FNab06217, 4ug; Detection:FNab06217 1:300) with HepG2 cells lysate 3000ug.

HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab06217(PCNA Antibody) at dilution of 1:10000