anti- PDI antibody

PRODUCT INFORMATION

Product Name

PDI antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).

IMMUNOGEN INFORMATION

Immunogen

prolyl 4-hydroxylase, beta polypeptide

Synonyms

DSI, ERBA2L, GIT, P4HB, P4Hbeta, p55, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, Protein disulfide isomerase

Observed MW

57kd

APPLICATION

Tested Application

ELISA, WB, IHC, IF, FC, IP

Recommended Dilution

WB: 1:500-1:1000; IP: 1:200-1:1000; IHC: 1:100-1:400; IF: 1:10-1:100

UNIPROT INFORMATION

UniProt ID

P07237

Immunofluorescent analysis of HEK-293 cells using FNab06272 (PDI antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

IP Result of anti-PDI (IP:FNab06272, 3ug; Detection:FNab06272 1:300) with mouse liver tissue lysate 4000ug.

COLO 320 cells were subjected to SDS PAGE followed by western blot with FNab06272(PDIA1 antibody) at dilution of 1:500