anti- PDK1 antibody

PRODUCT INFORMATION

Product Name

PDK1 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.

IMMUNOGEN INFORMATION

Immunogen

pyruvate dehydrogenase kinase, isozyme 1

Synonyms

PDH kinase 1, PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1

Observed MW

50kd

APPLICATION

Tested Application

ELISA, IHC, WB, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

Q15118

IP Result of anti-PDK1 (IP:FNab06275, 6ug; Detection:FNab06275 1:1000) with NIH/3T3 cells lysate 1200ug.

mouse liver tissue were subjected to SDS PAGE followed by western blot with FNab06275(PDK1 Antibody) at dilution of 1:1000