anti- PHD3 antibody

PRODUCT INFORMATION

Product Name

PHD3 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor(HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation(ODD) domains(N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.

IMMUNOGEN INFORMATION

Immunogen

egl nine homolog 3

Synonyms

Egl nine homolog 3, EGLN3, FLJ21620, HIF PH3, HIF prolyl hydroxylase 3, HIFPH3, HPH 1, HPH 3, PHD3

Observed MW

28kd

APPLICATION

Tested Application

ELISA, WB, IHC, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q9H6Z9

IP Result of anti-PHD3 (IP:FNab06377, 4ug; Detection:FNab06377 1:500) with A375 cells lysate 3600ug.

HT-1080 cells were subjected to SDS PAGE followed by western blot with FNab06377(EGLN3 antibody) at dilution of 1:1000