anti- PHF2 antibody

PRODUCT INFORMATION

Product Name

PHF2 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3(H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3(H3K4me3) at rDNA promoters and promotes expression of rDNA.

IMMUNOGEN INFORMATION

Immunogen

PHD finger protein 2

Synonyms

GRC5, JHDM1E, KIAA0662, PHD finger protein 2, PHF2

Observed MW

100 kDa, 150 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IP

Recommended Dilution

WB: 1:200-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

O75151

IP Result of anti-PHF2 (IP:FNab06384, 4ug; Detection:FNab06384 1:500) with HeLa cells lysate 1800ug.

Jurkat cells were subjected to SDS PAGE followed by western blot with FNab06384(PHF2 Antibody) at dilution of 1:500