anti- PI3K p85(alpha) antibody

PRODUCT INFORMATION

Product Name

PI3K p85(alpha) antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Phosphatidylinositol 3-kinase (PI3K) plays an important role in the metabolic actions of insulin and is required for adipogenesis. The PI3K pathway has also been identified as an important player in cancer development and progression. The Class IA PI3K heterodimer, which is composed of a P110 catalytic subunit and a P85 regulatory subunit, is activated upon association of the P85 subunit with upstream adaptor proteins or receptor tyrosine kinases. Mutations in PIK3R1 are implicated in cases of breast cancer and associated to SHORT syndrome.

IMMUNOGEN INFORMATION

Immunogen

phosphoinositide-3-kinase, regulatory subunit 1(alpha)

Synonyms

GRB1, p85, p85 ALPHA, PI 3 Kinase p85 alpha, PI 3-kinase p85α, PI3 kinase subunit p85 alpha, PI3K p85(alpha), PI3K regulatory subunit alpha, PIK3R1

Observed MW

85 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF, IP

Recommended Dilution

WB: 1:1000-1:10000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

P27986

Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using FNab06422(PIK3R1 Antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L)

IP Result of anti- PIK3R1 (IP: FNab06422, 5ug; Detection: FNab06422 1:500) with RAW 264.7 cells lysate 1480ug.

Neuro-2a cells were subjected to SDS PAGE followed by western blot with FNab06422(PIK3R1 Antibody) at dilution of 1:4000