anti- PKC gamma antibody

PRODUCT INFORMATION

Product Name

PKC gamma antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Protein kinase C(PKC) is a family of serine-and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14(SCA14), an autosomal dominant neurodegenerative disease.

IMMUNOGEN INFORMATION

Immunogen

protein kinase C, gamma

Synonyms

PKC gamma, PKCC, PKCG, PRKCG, Protein kinase C gamma type, protein kinase C, gamma, SCA14

Observed MW

78 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, FC, IP

Recommended Dilution

WB: 1:500-1:5000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

P05129

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using FNab06485( PRKCG Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

IP Result of anti-PKC gamma (IP:FNab06485, 4ug; Detection:FNab06485 1:500) with mouse brain tissue lysate 2640ug.

human brain tissue were subjected to SDS PAGE followed by western blot with FNab06485(PRKCG antibody) at dilution of 1:1000