anti- PKC iota antibody

PRODUCT INFORMATION

Product Name

PKC iota antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Calcium-and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis.

IMMUNOGEN INFORMATION

Immunogen

protein kinase C, iota

Synonyms

aPKC lambda/iota, DXS1179E, PKC iota, PKCI, PRKC lambda/iota, PRKCI, Protein kinase C iota type, protein kinase C, iota

Observed MW

68 kDa

APPLICATION

Tested Application

ELISA, IHC, WB, IP

Recommended Dilution

WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:50-1:500; IF: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

P41743

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using FNab06486 (PKC iota antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)

IP Result of anti-PKC iota (IP: FNab06486, 3ug; Detection: FNab06486 1:500) with HeLa cells lysate 2500ug.

HeLa cells were subjected to SDS PAGE followed by western blot with FNab06486(PRKCI antibody) at dilution of 1:500