anti- PKM2-specific antibody

anti- PKM2-specific antibody

Product Name

PKM2-specific antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

PKM2, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM2 plays a general role in caspase independent cell death of tumor cells. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells.The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2.(PMID:18337823) PKM2, Pyruvate kinase isozymes M1/M2, has 2 isoforms .The immunogen of this antibody is M2 isoform, also called PKM2. This antibody is specific to PKM2 isoform.

IMMUNOGEN INFORMATION

Immunogen

PKM2 pyruvate kinase, muscle

Synonyms

CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM2, PK-M2, PKM2 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK

Observed MW

58 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF, IP

Recommended Dilution

WB: 1:1000-1:4000; IP: 1:200-1:1000; IHC: 1:100-1:400; IF: 1:10-1:100

UNIPROT INFORMATION

UniProt ID

P14618

IMAGES

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using FNab06495(PKM2-specific Antibody) at dilution of 1:200

Immunofluorescent analysis of MCF-7 cells, using FNab06495(PKM2-specific Antibody) at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red)

IP Result of anti-PKM2-specific (IP:FNab06495, 4ug; Detection:FNab06495 1:300) with MCF-7 cells lysate 3250ug.

MCF7 cells were subjected to SDS PAGE followed by western blot with FNab06495(PKM2-specific antibody) at dilution of 1:1500