anti- POLI antibody

Product Name

POLI antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.

IMMUNOGEN INFORMATION

Immunogen

polymerase(DNA directed) iota

Synonyms

DNA polymerase iota, Eta2, POLI, polymerase (DNA directed) iota, RAD30 homolog B, RAD30B, RAD3OB

Observed MW

87 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q9UNA4

IP Result of anti-POLI (IP:FNab06612, 4ug; Detection:FNab06612 1:1000) with mouse testis tissue lysate 4000ug.

mouse testis tissue were subjected to SDS PAGE followed by western blot with FNab06612(POLI antibody) at dilution of 1:800