anti- POLR3K antibody

Product Name

POLR3K antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs(EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway(By similarity).

IMMUNOGEN INFORMATION

Immunogen

polymerase(RNA) III(DNA directed) polypeptide K, 12.3 kDa

Synonyms

C11, C11 RNP3, hRPC11, HsC11p, My010, POLR3K, RNA polymerase III subunit C10, RNA polymerase III subunit C11, RPC10, RPC11, RPC12.5

Observed MW

12 kDa

APPLICATION

Tested Application

ELISA, IHC, IF, WB

Recommended Dilution

WB: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

Q9Y2Y1

Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab06634(POLR3K Antibody) at dilution of 1:1000