anti- PPAR gamma antibody

Product Name

PPAR gamma antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.

IMMUNOGEN INFORMATION

Immunogen

peroxisome proliferator-activated receptor gamma

Synonyms

CIMT1, NR1C3, PPAR gamma, PPARG, PPARG1, PPARG2, PPARgamma, PPARγ

Observed MW

58 kDa

APPLICATION

Tested Application

ELISA, WB, IF, IHC, IP

Recommended Dilution

WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:10-1:100; IP: 1:500-1:1000

UNIPROT INFORMATION

UniProt ID

P37231

Immunofluorescent analysis of HepG2 cells using FNab06660 (PPAR gamma antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

IP Result of anti-PPAR gamma (IP:FNab06660, 3ug; Detection:FNab06660 1:700) with HL-60 cells lysate 4000ug.

K-562 cells were subjected to SDS PAGE followed by western blot with FNab06660(PPARG antibody) at dilution of 1:1000