anti- Pre-IL18 antibody

Product Name

Pre-IL18 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release interferon gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 24 kDa precursor and then cleaved into a biologically activate 18 kDa form. This antibody is raised against the full length IL18 precursor, and it recognizes both the pre-IL18 and mature IL18 theoretically.

IMMUNOGEN INFORMATION

Immunogen

interleukin 18(interferon-gamma-inducing factor)

Synonyms

Iboctadekin, IFN gamma inducing factor, IGIF, IL 1 gamma, IL 18, IL 1g, IL18, IL1F4, Interleukin 1 gamma, Interleukin 18, Mature IL18, pre IL18

Observed MW

24 kDa

APPLICATION

Tested Application

ELISA, WB, IP, IHC, IF

Recommended Dilution

WB: 1:500-1:2000; IP: 1:500-1:5000 ; IHC: 1:50-1:500; IF: 1:10-1:100

UNIPROT INFORMATION

UniProt ID

Q14116

Immunofluorescent analysis of HeLa cells using FNab06764 ( Pre-IL18 Antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

IP Result of anti-Pre-IL18 (IP:FNab06764, 3ug; Detection:FNab06764 1:1000) with HeLa cells lysate 5000ug.

HeLa cells were subjected to SDS PAGE followed by western blot with FNab06764(Pre-IL18 antibody) at dilution of 1:1000