anti- RBX1 antibody

anti- RBX1 antibody

Product Name

RBX1 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM.

IMMUNOGEN INFORMATION

Immunogen

ring-box 1

Synonyms

RNF75, ROC1

Observed MW

12 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P62877

IMAGES

Immunohistochemistry of paraffin-embedded human heart using FNab07199(RBX1 antibody) at dilution of 1:50

IP Result of anti-RBX1 (IP:FNab07199, 4ug; Detection:FNab07199 1:500) with HeLa cells lysate 1520ug.

HeLa cells were subjected to SDS PAGE followed by western blot with FNab07199(RBX1 antibody) at dilution of 1:500