anti- RECQL5 antibody

anti- RECQL5 antibody

Product Name

RECQL5 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Isoform beta is a DNA helicase that plays an important role in DNA replication, transcription and repair. Inhibits elongation of stalled transcripts at DNA damage sites by binding to the RNA polymerase II subunit POLR2A and blocking the TCEA1 binding site. Required for mitotic chromosome separation after cross-over events and cell cycle progress. Required for efficient DNA repair, including repair of inter-strand cross-links. Stimulates DNA decatenation mediated by TOP2A. Prevents sister chromatid exchange and homologous recombination.

IMMUNOGEN INFORMATION

Immunogen

RecQ protein-like 5

Synonyms

RECQL5, DNA helicase, RecQ like type 5, ATP dependent DNA helicase Q5, RecQ protein like 5, RECQ5

Observed MW

50-60 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF

Recommended Dilution

WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

O94762

IMAGES

Immunohistochemistry of paraffin-embedded human pancreas cancer using FNab07227(RECQL5 antibody) at dilution of 1:50

Immunofluorescent analysis of HepG2 cells, using RECQL5 antibody FNab07227 at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

mouse testis tissue were subjected to SDS PAGE followed by western blot with FNab07227(RECQL5 antibody) at dilution of 1:1000