anti- RFC4 antibody

anti- RFC4 antibody

Product Name

RFC4 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

BACKGROUND

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.

IMMUNOGEN INFORMATION

Immunogen

replication factor C (activator 1) 4, 37kDa

Observed MW

38 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF, IP

Recommended Dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:100; IP: 1:50 - 1:200

UNIPROT INFORMATION

UniProt ID

P35249

IMAGES

Immunohistochemistry of paraffin-embedded rat testis using FNab07250(RFC4 antibody) at dilution of 1:100

HeLa cells were subjected to SDS PAGE followed by western blot with FNab07250(RFC4 antibody) at dilution of 1:1000