anti- RICTOR antibody

anti- RICTOR antibody

Product Name

RICTOR antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.

IMMUNOGEN INFORMATION

Immunogen

rapamycin-insensitive companion of mTOR

Synonyms

AVO3 homolog, hAVO3, KIAA1999, mAVO3, RICTOR

Observed MW

192 kDa

APPLICATION

Tested Application

ELISA, WB, IHC

Recommended Dilution

WB: 1:1000-1:5000; IHC: 1:50-1:200

UNIPROT INFORMATION

UniProt ID

Q6R327

IMAGES

Immunohistochemistry of paraffin-embedded human lung cancer using FNab07297(RICTOR antibody) at dilution of 1:100

HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab07297(RICTOR antibody) at dilution of 1:4000