anti- RIP3 antibody

anti- RIP3 antibody

Product Name

RIP3 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.

IMMUNOGEN INFORMATION

Immunogen

receptor-interacting serine-threonine kinase 3

Synonyms

Receptor interacting protein 3, RIP 3, RIP like protein kinase 3, RIP3, RIPK3

Observed MW

57 kDa

APPLICATION

Tested Application

ELISA, IP, WB,IHC

Recommended Dilution

WB: 1:200-1:1000; IP: 1:200-1:1000; IHC: 1:100-1:400

UNIPROT INFORMATION

UniProt ID

Q9Y572

IMAGES

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using FNab07312(RIP3 antibody) at dilution of 1:200

IP Result of anti-RIP3 (IP: FNab07312, 3ug; Detection: FNab07312 1:300) with SW 1990 cells lysate 3000ug.

Jurkat cells were subjected to SDS PAGE followed by western blot with FNab07312(RIP3 antibody) at dilution of 1:500