anti- RNF20 antibody

anti- RNF20 antibody

Product Name

RNF20 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B(H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation(H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.

IMMUNOGEN INFORMATION

Immunogen

ring finger protein 20

Synonyms

BRE1A

Observed MW

114 kDa

APPLICATION

Tested Application

ELISA, WB, IF, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:200-1:1000; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q5VTR2

IMAGES

IP Result of anti-RNF20 (IP:FNab07349, 3ug; Detection:FNab07349 1:500) with HeLa cells lysate 2000ug.

HeLa cells were subjected to SDS PAGE followed by western blot with FNab07349(RNF20 antibody) at dilution of 1:500