anti- ROCK2 antibody

anti- ROCK2 antibody

Product Name

ROCK2 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

IMMUNOGEN INFORMATION

Immunogen

Rho-associated, coiled-coil containing protein kinase 2

Synonyms

KIAA0619, p164 ROCK 2, Rho kinase 2, ROCK2

Observed MW

161 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF, IP

Recommended Dilution

WB: 1:1000-1:10000; IP: 1:200-1:2000;; IHC: 1:20-1:200; IF: 1:10-1:100

UNIPROT INFORMATION

UniProt ID

O75116

IMAGES

Immunohistochemistry of paraffin-embedded human liver using FNab07378(ROCK2 antibody) at dilution of 1:100

IP Result of anti-ROCK2(middle) (IP:FNab07378, 5ug; Detection:FNab07378 1:700) with HeLa cells lysate 1320ug.

HT-1080 cells were subjected to SDS PAGE followed by western blot with FNab07378(ROCK2 antibody) at dilution of 1:1000