anti- RPL13A antibody

anti- RPL13A antibody

Product Name

RPL13A antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Associated with ribosomes but is not required for canonical ribosome function and has extra-ribosomal functions. Component of the GAIT(gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the ribosome and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs(such as ceruplasmin) and suppresses their translation. In the GAIT complex interacts with m7G cap-bound eIF4G at or near the eIF3-binding site and blocks the recruitment of the 43S ribosomal complex. Involved in methylation of rRNA.

IMMUNOGEN INFORMATION

Immunogen

ribosomal protein L13a

Observed MW

25-28 kDa

APPLICATION

Tested Application

ELISA, WB

Recommended Dilution

WB: 1:500-1:2000

UNIPROT INFORMATION

UniProt ID

P40429

IMAGES

HeLa cells were subjected to SDS PAGE followed by western blot with FNab07413(RPL13A antibody) at dilution of 1:400