anti- SHARPIN antibody

anti- SHARPIN antibody

Product Name

SHARPIN antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex(TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.

IMMUNOGEN INFORMATION

Immunogen

SHANK-associated RH domain interactor

Synonyms

SIPL1

Observed MW

40 kDa

APPLICATION

Tested Application

ELISA, WB, IP, IHC

Recommended Dilution

WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q9H0F6

IMAGES

Immunohistochemistry of paraffin-embedded human small intestine tissue slide using FNab07838(SHARPIN Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IP Result of anti-SHARPIN (IP:FNab07838, 3ug; Detection:FNab07838 1:1500) with Raji cells lysate 2520ug.

Raji cells were subjected to SDS PAGE followed by western blot with FNab07838(SHARPIN antibody) at dilution of 1:500