anti- SHP2 antibody

anti- SHP2 antibody

Product Name

SHP2 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

PTPN11(protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism(PMID:19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions(PMID:21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1(LEOPARD1), Noonan syndrome type 1(NS1), juvenile myelomonocytic leukemia(JMML) and metachondromatosis(MC). It has 3 isoforms produced by alternative splicing.

IMMUNOGEN INFORMATION

Immunogen

protein tyrosine phosphatase, non-receptor type 11

Synonyms

BPTP3, CFC, NS1, PTP 1D, PTP 2C, PTP2C, PTPN11, SH PTP2, SH PTP3, SHP 2, SHP2, SHPTP2

Observed MW

68kDa, 53kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF, IP

Recommended Dilution

WB: 1:500-1:5000; IP: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:10-1:100

UNIPROT INFORMATION

UniProt ID

Q06124

IMAGES

Immunohistochemistry of paraffin-embedded human breast cancer using FNab07854(PTPN11 antibody) at dilution of 1:100

Immunofluorescent analysis of Hela cells, using FNab07854(PTPN11 antibody)at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

IP Result of anti-SHP2 (IP:FNab07854, 4ug; Detection:FNab07854 1:1000) with HEK-293 cells lysate 2800ug.

Jurkat cells were subjected to SDS PAGE followed by western blot with FNab07854(PTPN11 antibody) at dilution of 1:500