anti- SOCS3 antibody

anti- SOCS3 antibody

Product Name

SOCS3 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo(By similarity). Probable substrate recognition component of a SCF-like ECS(Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST(By similarity).

IMMUNOGEN INFORMATION

Immunogen

suppressor of cytokine signaling 3

Synonyms

ATOD4, CIS 3, CIS3, Cish3, SOCS 3, SOCS3, SSI 3, SSI3, STAT induced STAT inhibitor 3

Observed MW

30 kDa

APPLICATION

Tested Application

ELISA, IHC, WB, IF, IP

Recommended Dilution

WB: 1:200-1:2000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:10-1:100

UNIPROT INFORMATION

UniProt ID

O14543

IMAGES

Immunohistochemistry of paraffin-embedded human heart using FNab08100(SOCS3 antibody) at dilution of 1:50

Immunofluorescent analysis of HepG2 cells, using FNab08100(SOCS3 antibody)at 1:10 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

IP Result of anti-SOCS3 (IP:FNab08100,4ug; Detection: FNab08100 1:500) with K-562 cells lysate 1720ug.

K-562 cells were subjected to SDS PAGE followed by western blot with FNab08100(SOCS3 antibody) at dilution of 1:500