anti- SPAG8 antibody

anti- SPAG8 antibody

Product Name

SPAG8 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Plays a role in spermatogenesis by enhancing the binding of CREM isoform tau to its coactivator FHL5 and increasing the FHL5-regulated transcriptional activation of CREM isoform tau(By similarity). Involved in the acrosome reaction and in binding of sperm to the zona pellucida(By similarity). Plays a role in regulation of the cell cycle by controlling progression through the G2/M phase, possibly by delaying the activation of CDK1 which is required for entry into mitosis(PubMed:19548270). May play a role in fertility and microtubule formation through interaction with RANBP9(PubMed:10500252).

IMMUNOGEN INFORMATION

Immunogen

sperm associated antigen 8

Observed MW

56-65 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IP

Recommended Dilution

WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q99932

IMAGES

Immunohistochemistry of paraffin-embedded human prostate cancer using FNab08146(SPAG8 antibody) at dilution of 1:100

IP Result of anti-SPAG8 (IP:FNab08146, 3ug; Detection:FNab08146 1:500) with mouse testis tissue lysate 6000ug.

PC-3 cells were subjected to SDS PAGE followed by western blot with FNab08146(SPAG8 antibody) at dilution of 1:1000