anti- SQLE antibody

anti- SQLE antibody

Product Name

SQLE antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

SQLE, also named as ERG1, SE and SM, belongs to the squalene monooxygenase family. It catalyzes the first oxygenation step in cholesterol synthesis, acting on squalene before cyclization into the basic steroid structure. SQLE may serve as a flux-controlling enzyme beyond 3-hydroxy-3-methylglutaryl-coenzyme A reductase(HMGR, considered as rate limiting). It is also posttranslationally regulated by cholesterol-dependent proteasomal degradation.(PMID:21356516) The MW of SQLE is about 64kd.

IMMUNOGEN INFORMATION

Immunogen

squalene epoxidase

Synonyms

ERG1, FLJ30795, SE, SM, SQLE, squalene epoxidase, Squalene monooxygenase

Observed MW

64 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IP, IF

Recommended Dilution

WB: 1:200-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

Q14534

IMAGES

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab08209(SQLE Antibody) at dilution of 1:50

IP Result of anti-SQLE (IP:FNab08209, 3ug; Detection:FNab08209 1:300) with A549 cells lysate 920ug.

A549 cells were subjected to SDS PAGE followed by western blot with FNab08209(SQLE antibody) at dilution of 1:300