anti- SRC antibody

anti- SRC antibody

Product Name

SRC antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.

IMMUNOGEN INFORMATION

Immunogen

v-src sarcoma(Schmidt-Ruppin A-2) viral oncogene homolog

Synonyms

ASV, c SRC, p60 Src, pp60c src, Proto oncogene c Src, SRC, SRC1

Observed MW

55kd,60kd

APPLICATION

Tested Application

ELISA, IHC, IP, FC, IF, WB

Recommended Dilution

WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:100-1:400; IF: 1:10-1:100

UNIPROT INFORMATION

UniProt ID

P12931

IMAGES

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab08216(c-SRC Antibody) at dilution of 1:200

Immunofluorescent analysis of A431 cells using FNab08216 (SRC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)

IP Result of anti-SRC (IP: FNab08216, 5ug; Detection: FNab08216 1:1000) with SH-SY5Y cells lysate 2900ug.

Jurkat cells were subjected to SDS PAGE followed by western blot with FNab08216(SRC antibody) at dilution of 1:1000