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FNab08977

anti- TRIM27 antibody

anti- TRIM27 antibody

$310.00 USD
$310.00 USD
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size

anti- TRIM27 antibody

Product Name

TRIM27 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASH1, leading to promote endosomal F-actin assembly(PubMed:23452853). Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA.

IMMUNOGEN INFORMATION

Immunogen

tripartite motif-containing 27

Synonyms

Ret finger protein, RFP, RING finger protein 76, RNF76, TRIM27, tripartite motif containing 27, Zinc finger protein RFP

Observed MW

58 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IP, IF

Recommended Dilution

WB: 1:500-1:2000; IP: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:20-1:200

UNIPROT INFORMATION

UniProt ID

P14373

IMAGES

 

Immunohistochemistry of paraffin-embedded human lung cancer using FNab08977(TRIM27 antibody) at dilution of 1:100
Immunofluorescent analysis of HeLa cells using FNab08977 (TRIM27 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
IP Result of anti-TRIM27 (IP:FNab08977, 4ug; Detection:FNab08977 1:1000) with mouse testis tissue lysate 4000ug.
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab08977(TRIM27 antibody) at dilution of 1:1000

 

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